After two long days of debate in the Scottish Parliament, the controversial Gender Recognition Reform (Scotland) Bill has been passed by 86 votes to 39.

The bill means:

• The minimum age at which someone can apply for a gender recognition certificate (GRC) will be lowered to 16 from 18.

• There will no longer be a need for a medical diagnosis of gender dysphoria in order to receive a GRC.

• The time required for an applicant to live in their acquired gender will be cut from two years to three months with a three-month reflection period (six months for 16 and 17-year-olds).

The bill has proved to be one of the most controversial in Holyrood since devolution but passed following support from many in the SNP, Greens, Labour and Liberal Democrats.

Protesters took part in demonstrations both for and against the bill outside Holyrood on Wednesday as MSPs went through the final amendments.

Opponents have raised concerns over its impact on the safety of women and girls, arguing the bill should not have been passed as it fails to protect women's rights and single-sex spaces.

The UN's special rapporteur on violence against women and girls said the reforms could allow violent males to "abuse" the system.

But the Scottish government has insisted the legislation will not impact the Equality Act, which allows trans people to be excluded from single-sex spaces such as changing rooms and shelters.

The bill also proved controversial as it means the laws in Scotland will now be different to the rest of the UK.

More than 60 of the 153 amendments were voted on in the first sitting on Tuesday, with the rest in the day after.

There were protests from the public gallery as opponents shouted "shame on you" when an amendment that would make it harder for sex offenders to apply for a GRC was voted down.

The Scottish Conservatives appeared to be attempting to make the proceedings last as long as possible, tabling four amendments, a motion and a number of points of order before the debate had started - meaning the sitting on Tuesday finished at 12.15am.

While First Minister Nicola Sturgeon has been a strong supporter of the bill, not all in the SNP have toed the line, with seven MSPs from the ruling party voting against it and two others abstaining at stage one.

Minister Ash Regan was forced to quit over her objection.

MSPs backed a change on Tuesday which means anyone subject to a sexual harm prevention order or sexual offences prevention order will not be allowed to obtain a GRC.

An amendment to ensure applications are paused if an applicant is charged with a sexual offence until their case is disposed of divided members evenly, with the deputy presiding officer voting against it so it was not added to the bill.